Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

3.4K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
3.4K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

3.1K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathogenic CD8+ T cells target K71+ Henle's layer by forming cytolytic immune synapses in Alopecia Areata.

Research square·2026
Same author

IL-1 signaling enrichment in inflammatory skin disease loci with higher-risk allele frequencies in African ancestry.

Research square·2025
Same author

Meeting Report on "The International Congress on Autoimmune Pre-disease (2024)".

JID innovations : skin science from molecules to population health·2025
Same author

A scalable and cGMP-compatible autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa.

Nature communications·2024
Same author

Cytosine Deaminase Base Editing to Restore <i>COL7A1</i> in Dystrophic Epidermolysis Bullosa Human: Murine Skin Model.

JID innovations : skin science from molecules to population health·2023
Same author

A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa.

bioRxiv : the preprint server for biology·2023

Related Experiment Video

Updated: Mar 1, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

13.3K

Next generation human skin constructs as advanced tools for drug development.

H E Abaci1, Zongyou Guo1, Yanne Doucet1

  • 11 Department of Dermatology, Columbia University Medical Center, New York, NY 10032, USA.

Experimental Biology and Medicine (Maywood, N.J.)
|June 9, 2017
PubMed
Summary

Developing advanced in vitro skin models using induced pluripotent stem cells (iPSC) and microfabrication is crucial for drug discovery and disease research. These biomimetic models aim to replicate complex skin functions for more accurate pharmaceutical screening.

Keywords:
Skin constructsdrug testingmicrophysiological systemsskin-on-a-chip

More Related Videos

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform
06:30

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

Published on: May 17, 2021

4.9K
Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System
04:37

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Published on: October 17, 2025

2.4K

Related Experiment Videos

Last Updated: Mar 1, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

13.3K
Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform
06:30

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

Published on: May 17, 2021

4.9K
Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System
04:37

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Published on: October 17, 2025

2.4K

Area of Science:

  • Tissue Engineering
  • Biomaterials Science
  • Stem Cell Biology

Background:

  • Skin diseases and drug side effects often present with cutaneous symptoms.
  • Engineering functional in vitro skin models is challenging due to complex anatomy and cell types.
  • Previous models lacked key physiological components like vasculature and appendages.

Purpose of the Study:

  • To review advancements in creating complex, biomimetic in vitro human skin constructs.
  • To highlight the role of induced pluripotent stem cell (iPSC) technology and microfabrication.
  • To discuss challenges and future directions in skin modeling for drug discovery and regenerative medicine.

Main Methods:

  • Utilizing induced pluripotent stem cell (iPSC) technology for diverse skin cell generation.
  • Employing microfabrication techniques, including 3D-printing, for construct development.
  • Integrating various skin components: vasculature, appendages, pigmentation, immune cells, and innervation.

Main Results:

  • Progress in generating skin constructs with vasculature, appendages, and pigmentation.
  • Development of in vitro models for genetic skin diseases like epidermolysis bullosa and psoriasis.
  • Potential for patient-specific drug testing through advanced skin models.

Conclusions:

  • Biomimetic in vitro skin models are essential tools for pharmaceutical screening and disease modeling.
  • iPSC technology and microfabrication are key enablers for complex skin construct development.
  • Future integration with microfluidic platforms will revolutionize drug development and personalized medicine.