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Published on: July 11, 2015
Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results
W Ray Waters1, H Martin Vordermeier2, Shelley Rhodes2
1National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture (USDA), Ames, IA, USA.
Background:
Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle.
Results:
Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB.
Conclusions:
Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.
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