Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.7K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.7K
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

2.2K
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A generalised modelling approach to predict retention in surfactant-mediated liquid chromatographic modes.

Journal of chromatography. A·2025
Same author

Implementation of generalised retention models in RPLC using serially coupled columns: a study based on lemon balm extracts.

Analytica chimica acta·2025
Same author

Description of chromatographic performance in reversed-phase liquid chromatography based on a generalised model. Part I: Column characterisation.

Journal of chromatography. A·2025
Same author

Description of chromatographic performance in reversed-phase liquid chromatography based on a generalised model. Part II: Prediction of serial-column coupling.

Journal of chromatography. A·2025
Same author

Global retention model based on multisample system parameters for optimising chromatographic fingerprints of medicinal plants.

Analytica chimica acta·2025
Same author

Transferability of global retention models in reversed-phase liquid chromatography for natural products.

Journal of chromatography. A·2024

Related Experiment Video

Updated: Mar 1, 2026

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
06:03

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

Published on: June 23, 2023

870

Assisted baseline subtraction in complex chromatograms using the BEADS algorithm.

J A Navarro-Huerta1, J R Torres-Lapasió1, S López-Ureña2

  • 1Department of Analytical Chemistry, Faculty of Chemistry, Universitat de València, c/ Dr. Moliner 50, 46100 Burjassot, Spain.

Journal of Chromatography. A
|June 9, 2017
PubMed
Summary
This summary is machine-generated.

A new assisted tool for Baseline Estimation And Denoising using Sparsity (BEADS) streamlines high-performance liquid chromatography data processing. This method enhances speed and reliability in baseline subtraction for complex chromatograms.

Keywords:
AutocorrelationBEADSBaseline subtractionComplex samplesHPLCWorking parameters

More Related Videos

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.5K
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

12.0K

Related Experiment Videos

Last Updated: Mar 1, 2026

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
06:03

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

Published on: June 23, 2023

870
Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.5K
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

12.0K

Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Signal Processing

Background:

  • Data processing in high-performance liquid chromatography (HPLC) can be a bottleneck for extracting meaningful information from chromatograms.
  • Automated and efficient data pre-processing is crucial for maximizing analytical benefits and processing speed.
  • Conventional baseline subtraction algorithms often rely on slow and inconsistent trial-and-error parameter selection.

Purpose of the Study:

  • To develop and verify an assisted configuration tool for the BEADS (Baseline Estimation And Denoising using Sparsity) algorithm.
  • To improve the speed, reliability, and user-friendliness of baseline subtraction in HPLC data analysis.
  • To introduce a quality criterion for automated selection of optimal working parameters for BEADS.

Main Methods:

  • Development of an assisted configuration tool for the BEADS algorithm.
  • Implementation of a quality criterion based on autocorrelation level for parameter selection.
  • Application of log transformation to the signal to mitigate artifacts from large signal disparities.
  • Testing the assisted BEADS algorithm on complex HPLC chromatograms of medicinal herb extracts and sulphonamide mixtures.

Main Results:

  • The assisted BEADS algorithm significantly simplifies parameter setup compared to conventional methods.
  • Processing time for baseline subtraction is substantially shortened.
  • The reliability of baseline subtraction is improved, leading to more accurate data analysis.
  • The developed quality criterion effectively identifies optimal parameters for accurate baseline estimation.

Conclusions:

  • The assisted BEADS tool offers a faster, more reliable, and less user-dependent approach to baseline subtraction in HPLC.
  • This advancement addresses a key bottleneck in processing complex chromatographic data.
  • The method is validated on diverse and challenging sample types, demonstrating its practical utility.