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Updated: Mar 1, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Functionalization of natural compounds by enzymatic fructosylation
Azucena Herrera-González1,2, Gema Núñez-López1,2, Sandrine Morel2
1Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. - Unidad Zapopan, Camino Arenero 1227, El Bajio del Arenal, 4519, Zapopan, JAL, Mexico.
Abstract:
Enzymatic fructosylation of organic acceptors other than sugar opens access to the production of new molecules that do not exist in nature. These new glycoconjugates may have improved physical-chemical and bioactive properties like solubility, stability, bioavailability, and bioactivity. This review focuses on different classes of acceptors including alkyl alcohols, aromatic alcohols, alkaloids, flavonoids, and xanthonoids, which were tested for the production of fructoderivatives using enzymes from the glycoside hydrolase (GH) families 32 and 68 that use sucrose as donor substrate. The enzymatic strategies and the reaction conditions required for the achievement of these complex reactions are discussed, in particular with regard to the type of acceptors. The solubility and pharmacokinetic and antioxidant activity of some of these new β-D-fructofuranosides in comparison is reviewed and compared with their glucoside analogs to highlight the differences between these molecules for technological applications.
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