A Sensitive Photometric Procedure for Cobalt Determination in Water Employing a Compact Multicommuted Flow Analysis...

In this work, a multicommuted flow analysis procedure is proposed for the spectrophotometric determination of cobalt in fresh water, employing an instrument setup of downsized dimension and improved cost-effectiveness. The method is based on the catalytic effect of Co(II) on the Tiron oxidation by hydrogen peroxide in alkaline medium, forming a complex that absorbs radiation at 425 nm. The photometric detection was accomplished using a homemade light-emitting-diode (LED)-based photometer designed to use a flow cell with an optical path-length of 100 mm to improve sensitivity. After selecting adequate values for the flow system variables, adherence to the Beer-Lambert-Bouguer law was observed for standard solution concentrations in the range of 0.13-1.5 µg L^-1 Co(II). Other useful features including a relative standard deviation of 2.0% (n = 11) for a sample with 0.49 µg L^-1 Co(II), a detection limit of 0.06 µg L^-1 Co(II) (n = 20), an analytical frequency of 42 sample determinations per hour, and waste generation of 1.5 mL per determination were achieved.