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Dimerization of GTR1 regulates their plasma membrane localization
Yasuhiro Ishimaru1, Kento Washiyama1, Takaya Oikawa1
1a Department of Chemistry , Tohoku University , Aoba-ku , Sendai , Japan.
Plant Signaling & Behavior
|June 9, 2017
Summary
Plant hormone transporter GTR1 (NPF2.10) dimerization, potentially triggered by dephosphorylation, regulates its plasma membrane localization. This dimerization controls the transport of glucosinolates and gibberellic acid, impacting plant growth.
Area of Science:
- Plant molecular biology
- Membrane transport
- Plant hormone signaling
Background:
- Nitrate transporter 1/peptide transporter family (NPF) proteins are crucial for plant growth and stress response, transporting diverse molecules including plant hormones.
- Arabidopsis GTR1 (NPF2.10) is known to transport glucosinolates, gibberellic acid, and jasmonoyl-L-isoleucine.
- While GTR1 expression is regulated, post-translational modifications controlling its activity remain largely unexplored.
Purpose of the Study:
- To investigate the post-translational regulation of the multifunctional transporter GTR1.
- To determine if GTR1 dimerization affects its plasma membrane localization and transport activity.
Main Methods:
- Functional analysis of GTR1 in Xenopus oocytes.
- Investigation of GTR1 dimerization and its correlation with dephosphorylation.
- Assessment of plasma membrane localization of GTR1 under different conditions.
Main Results:
- GTR1 dimerization, possibly induced by dephosphorylation of a threonine residue, regulates its localization to the plasma membrane.
- This regulated localization is essential for the transport of glucosinolates and gibberellic acid.
- Evidence suggests dimerization is a key mechanism controlling the activity of multifunctional transporters.
Conclusions:
- Post-translational regulation, specifically dimerization, plays a significant role in controlling GTR1 function.
- Dimerization of GTR1 impacts its plasma membrane localization and transport capabilities for key molecules.
- These findings highlight dimerization as a critical regulatory mechanism for multifunctional transporters in plants.
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