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Related Experiment Video

Updated: Mar 1, 2026

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Differential expression between "DSP-only" and DSP-PP523 transcripts in rat molar teeth.

Ya-Qin Zhu1, Ryan M Song2, Helena H Ritchie2

  • 1Department of General Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.

Archives of Oral Biology
|June 9, 2017
PubMed
Summary
This summary is machine-generated.

The DSP-only transcript and DSP-PP523 transcript show different expression patterns during tooth development. DSP-only transcript is found in the dental pulp and below mature odontoblasts, suggesting distinct roles in dentinogenesis.

Keywords:
DSP-PP(523) transcriptDSP-only transcriptDifferential expressiona cell layer subjacent to odontoblast layer

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Area of Science:

  • Biochemistry
  • Developmental Biology
  • Genetics

Background:

  • The dentin sialophosphoprotein (DSPP) gene yields multiple transcripts, including DSP-only and DSP-PP523.
  • Understanding the differential expression of these transcripts is crucial for elucidating their specific roles in tooth development.

Purpose of the Study:

  • To compare the expression patterns of the DSP-only and DSP-PP523 transcripts during molar tooth development.
  • To investigate the potential distinct functions of these transcripts in dentinogenesis.

Main Methods:

  • Utilized in situ hybridization with unique riboprobes to detect DSP-only and DSP-PP523 transcript expression in developing rat molars.
  • Analyzed paraffin-embedded molar sections from various embryonic and postnatal stages.
  • Employed immunohistochemistry to identify Stro-1 protein expression, a stem cell marker.

Main Results:

  • DSP-PP523 transcript expression was observed in mature odontoblasts involved in dentin formation.
  • DSP-only transcript was detected in the dental pulp and subjacent to the mature odontoblast layer, not within the odontoblasts themselves.
  • Stro-1 protein, a stem cell marker, was also found in the dental pulp and areas subjacent to odontoblasts.

Conclusions:

  • The differential expression patterns of DSP-only and DSP-PP523 transcripts suggest they have distinct functions during dentinogenesis.
  • DSP-PP523 transcript is associated with active dentin formation by mature odontoblasts.
  • DSP-only transcript may play a different role, potentially related to stem cell populations in the dental pulp.