Related Experiment Video
Updated: Feb 28, 2026
07:34
Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
Published on: May 5, 2023
5.2K
GmTIR1/GmAFB3-based auxin perception regulated by miR393 modulates soybean nodulation
Zhaoming Cai1,2, Youning Wang1, Lin Zhu1
1State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
The New Phytologist
|June 10, 2017
Summary
Soybean nodulation involves auxin signaling. This study reveals that auxin receptors (GmTIR1/GmAFB3), regulated by miR393, are crucial for determinate nodule development and rhizobial infection.
Area of Science:
- Plant Molecular Biology
- Plant-Microbe Interactions
- Legume Symbiosis
Background:
- Auxins are vital for legume nodulation, but the precise mechanisms of auxin signaling in root nodule development are not fully understood.
- The specific roles of auxin receptors and their regulatory pathways in determinate nodule formation remain largely unelucidated.
Purpose of the Study:
- To investigate the expression patterns of soybean auxin receptor genes (GmTIR1/GmAFB3) during nodulation.
- To elucidate the functions of GmTIR1/AFB3 and miR393 in regulating rhizobial infection and nodule number.
- To determine the relationship between miR393 and GmTIR1/AFB3 in soybean nodulation.
Main Methods:
- Analyzed the expression patterns of GmTIR1/GmAFB3 genes in soybean under nodulation conditions.
- Performed gene overexpression and knockdown experiments for GmTIR1/AFB3 and miR393 family members.
- Utilized a mutated GmTIR1C construct resistant to miR393 cleavage to assess regulatory mechanisms.
Main Results:
- GmTIR1 and GmAFB3 genes displayed varied expression during soybean nodulation.
- Overexpression of GmTIR1 significantly enhanced rhizobial infection foci and nodule numbers.
- miR393 directly targets GmTIR1/AFB3, and its knockdown led to increased infection and nodule formation; a miR393-resistant GmTIR1C mutant further boosted these parameters.
Conclusions:
- GmTIR1- and GmAFB3-mediated auxin signaling plays a critical role in determinate nodule development in soybean.
- Spatio-temporal regulation of GmTIR1/AFB3 by miR393 is a key mechanism modulating soybean nodulation.
Related Concept Videos
01:25Cell Signaling in Plants
6.8K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.8K
02:35Key Elements for Plant Nutrition
24.6K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
24.6K
01:56Plant Hormones
27.9K
Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
27.9K