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Updated: Feb 28, 2026
Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
[Construction and Identification of the cDNA Expression Library for Human Esophageal Cancer Cells]
Zhe Zhang1, Xiang-Yu Wu2, Lu Feng2
1Department of Traditional Chinese Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061,China.
Objectives:
To construct a cDNA phage expression library for human esophageal cancer cells.
Methods:
After the total RNA were obtained from esophageal cancer cells, the mRNA were separated with magnetic beads adsorption method, and the single-strand and double-strand cDNA were synthesized through reverse transcription. With the undesirable cDNA fragments removed, the remaining cDNA (linked withEcoR1 aptamer and phosphorylated its 5'end) combined with the carrier of T7 Select10-3b. The recombinant phage were packaged in vitro for preliminary cDNA library. PCR was used to identify the size of inserted cDNA.
Results:
The constructed original cDNA phage expression library for human esophageal cancer cells was consisted of 2.01×10⁶ pfu/mL bacteriophages with a recombination rate of 100%. The length of the inserted cDNA fragments were range from 300 bp to 1 500 bp.
Conclusions:
The cDNA phage expression library of human esophageal cell is successfully constructed to meet the currently recognized standards, and can be well used to screen cDNA-cloned genes of human esophageal cancer antigens by serological analysis of recombinantly expressed cDNA clone (SEREX).
