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Published on: December 31, 2017
[Microbial Community Structure on the Root Surface of Patients with Periodontitis.]
Ju-Mei Zhang1, Jian-Ye Zhou1, Lei Bo2
1Key Laboratory of Oral Diseases of Gansu Province,Northwest University for Nationalities;Key Laboratory of Stomatology of State Ethnic Affairs Commission,Northwest University for Nationalities;School of Stomatology,Northwest University For Nationalities,Lanzhou 730030,China.
Objectives:
To study the microbial community structure on the root surface of patients with periodontitis.
Methods:
Bacterial plaque and tissues from the root neck (RN group),root middle (RM group) and root tine (RT group) of six teeth with mobility 3 in one patient with periodontitis were sampled.The V3V4 region of 16S rRNA was sequenced on the Illumina MiSeq platform.The microbial community structure was analyzed by Mothur,Qiime and SPSS software.
Results:
The principal component analysis (PCoA) results indicated that the RM samples had a similar microbial community structure as that of the RT samples,which was significant different from that of the RN samples.Thirteen phyla were detected in the three groups of samples,which included 7 dominant phyla.29 dominant genera were detected in 184 genera.The abundance of Bacteroidetes_[G-6] and Peptostre ptococcaceae_[XI][G-4] had a positive correlation with the depth of the collection site of samples (P<0.05),while the abundance of Prevotella,Selenomonas,Corynebacterium and Olsenella had a negative correlation with the depth of the collection site of samples (P<0.05).
Conclusions:
There is region-specificity of microbial community structure on the root surface of patients with periodontitis.
