Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

11.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.2K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

4.1K
4.1K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

13.0K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
13.0K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.7K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.7K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

3.1K
3.1K
Transcription Factors02:16

Transcription Factors

83.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
83.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The plusses and minuses of DNA torsion.

eLife·2025
Same author

Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates.

Science advances·2023
Same author

Dissecting transcriptional amplification by MYC.

eLife·2020
Same author

Controlling gene expression by DNA mechanics: emerging insights and challenges.

Biophysical reviews·2017
Same author

Controlling gene expression by DNA mechanics: emerging insights and challenges.

Biophysical reviews·2017
Same author

Tuning the MYC response.

eLife·2016
Same journal

Restraint of melanoma progression by cells in the local skin environment.

eLife·2026
Same journal

Brawn before bite in endemic Asian eutherian mammals after the end-Cretaceous extinction.

eLife·2026
Same journal

Experimental evolution to thermal stress indicates climate resilience in a cosmopolitan arthropod.

eLife·2026
Same journal

Correlates of protection against African swine fever virus identified by a systems immunology approach.

eLife·2026
Same journal

Retrosplenial cortex enables context-dependent goal-directed sensorimotor transformation.

eLife·2026
Same journal

Direct contact between iPSC-derived macrophages and hepatocytes drives reciprocal acquisition of Kupffer cell identity and hepatocyte maturation.

eLife·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers
08:12

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers

Published on: July 18, 2025

746

Enhancers not required.

Ying Zheng1, David Levens1

  • 1Laboratory of Pathology, National Cancer Institute, Bethesda, United States.

Elife
|June 10, 2017
PubMed
Summary
This summary is machine-generated.

Laboratory mice lacking over half a megabase of DNA upstream of their Myc gene can still thrive. This suggests significant genetic flexibility in Myc gene regulation, even without external stressors.

Keywords:
cancercancer biologyenhancerhumanmouseoncogene regulation

More Related Videos

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
03:34

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

391
A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

609

Related Experiment Videos

Last Updated: Feb 28, 2026

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers
08:12

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers

Published on: July 18, 2025

746
Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
03:34

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

391
A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

609

Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • The Myc gene is a critical regulator of cell proliferation, differentiation, and apoptosis.
  • Regulatory elements upstream of genes play crucial roles in controlling gene expression.
  • Understanding the impact of large-scale genomic deletions on gene regulation is essential for comprehending genetic stability and function.

Purpose of the Study:

  • To investigate the functional consequences of deleting a large DNA region upstream of the Myc gene in a mammalian model.
  • To assess the viability and physiological status of mice with significant genomic alterations near the Myc locus.
  • To determine the role of extensive upstream regulatory regions in Myc gene expression and organismal health under normal conditions.

Main Methods:

  • Generation of genetically modified laboratory mice with a large deletion (over 500 kilobases) upstream of the Myc gene.
  • Phenotypic analysis of the modified mice, including assessment of growth, development, and overall health.
  • Evaluation of Myc gene expression levels and downstream effects in various tissues (though not explicitly stated in the abstract, this would be a standard follow-up).

Main Results:

  • Mice with the large upstream DNA deletion demonstrated normal thriving and development.
  • Absence of overt physiological stress or developmental abnormalities was observed in the modified mice.
  • The removal of a substantial regulatory region did not impair the mice's ability to survive and thrive under standard laboratory conditions.

Conclusions:

  • A large segment of DNA upstream of the Myc gene is not essential for survival and normal thriving in laboratory mice.
  • This finding highlights a remarkable plasticity or redundancy in the regulatory mechanisms controlling Myc gene expression.
  • The results suggest that other regulatory elements or compensatory mechanisms may maintain adequate Myc function even after extensive genomic alteration in the absence of stress.