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Updated: Feb 28, 2026

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers
08:12

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Published on: July 18, 2025

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Enhancers not required.

Ying Zheng1, David Levens1

  • 1Laboratory of Pathology, National Cancer Institute, Bethesda, United States.

Elife
|June 10, 2017
PubMed
Summary
This summary is machine-generated.

Laboratory mice lacking over half a megabase of DNA upstream of their Myc gene can still thrive. This suggests significant genetic flexibility in Myc gene regulation, even without external stressors.

Keywords:
cancercancer biologyenhancerhumanmouseoncogene regulation

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Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • The Myc gene is a critical regulator of cell proliferation, differentiation, and apoptosis.
  • Regulatory elements upstream of genes play crucial roles in controlling gene expression.
  • Understanding the impact of large-scale genomic deletions on gene regulation is essential for comprehending genetic stability and function.

Purpose of the Study:

  • To investigate the functional consequences of deleting a large DNA region upstream of the Myc gene in a mammalian model.
  • To assess the viability and physiological status of mice with significant genomic alterations near the Myc locus.
  • To determine the role of extensive upstream regulatory regions in Myc gene expression and organismal health under normal conditions.

Main Methods:

  • Generation of genetically modified laboratory mice with a large deletion (over 500 kilobases) upstream of the Myc gene.
  • Phenotypic analysis of the modified mice, including assessment of growth, development, and overall health.
  • Evaluation of Myc gene expression levels and downstream effects in various tissues (though not explicitly stated in the abstract, this would be a standard follow-up).

Main Results:

  • Mice with the large upstream DNA deletion demonstrated normal thriving and development.
  • Absence of overt physiological stress or developmental abnormalities was observed in the modified mice.
  • The removal of a substantial regulatory region did not impair the mice's ability to survive and thrive under standard laboratory conditions.

Conclusions:

  • A large segment of DNA upstream of the Myc gene is not essential for survival and normal thriving in laboratory mice.
  • This finding highlights a remarkable plasticity or redundancy in the regulatory mechanisms controlling Myc gene expression.
  • The results suggest that other regulatory elements or compensatory mechanisms may maintain adequate Myc function even after extensive genomic alteration in the absence of stress.