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Gas Chromatography–Mass Spectrometry (GC–MS)

Gas Chromatography–Mass Spectrometry (GC–MS)

Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....

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Determination of reference intervals for urinary steroid profiling using a newly validated GC-MS/MS method.

Wilhelmina H A de Jong¹, Edward Buitenwerf¹, Alle T Pranger¹

¹.

Clinical Chemistry and Laboratory Medicine

|June 10, 2017

Summary

Urinary steroid profiling (USP) reference intervals should account for age, sex, and oral contraceptive pill (OCP) use. A new GC-MS/MS method provides validated intervals for 33 metabolites, improving diagnostic accuracy.

Keywords:

GC-MS/MS oral contraceptive pills reference intervals urinary steroid profile

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Area of Science:

Endocrinology
Clinical Chemistry
Mass Spectrometry

Background:

Urinary steroid profiling (USP) is crucial for diagnosing steroidogenesis disorders.
Current USP reference intervals often lack adjustments for key pre-analytical factors like age, sex, and oral contraceptive pill (OCP) use.
These factors can significantly influence steroid hormone synthesis and metabolism.

Purpose of the Study:

To establish age- and sex-specific reference intervals for USP using a novel gas chromatography-tandem mass spectrometry (GC-MS/MS) method.
To investigate the impact of OCP use on urinary steroid metabolite excretion.
To validate the developed GC-MS/MS assay for high-throughput USP analysis.

Main Methods:

A validated GC-MS/MS assay involving hydrolysis, solid-phase extraction, and double derivatization was employed.
Two hundred forty healthy individuals (20-79 years, stratified by decade and sex) were analyzed.
Forty women (20-39 years) using OCP were included to assess their impact.

Main Results:

Excretion of androgen metabolites decreased with age in both sexes.
Cortisol metabolite levels were stable with age, except for an increase in older women (70-79 years).
Progesterone metabolite excretion peaked in women aged 30-39 years. OCP use was associated with lower excretion of androgen, progesterone, and cortisol metabolites.

Conclusions:

A robust and validated GC-MS/MS method for USP analysis was developed.
Age- and sex-specific reference intervals for 33 urinary steroid metabolites and diagnostic ratios were established.
Reference intervals for USP should incorporate adjustments for age, sex, and OCP usage for improved diagnostic utility.