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Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
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Interleukin-24 inhibits osteosarcoma cell migration and invasion via the JNK/c-Jun signaling pathways

Baobiao Zhuo1, Yingchun Shi1, Haihui Qin1

  • 1Department of Surgery, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221006, P.R. China.

Oncology Letters
|June 11, 2017
PubMed

Insights

Interleukin-24 (IL-24) effectively suppresses osteosarcoma metastasis by inhibiting cell migration and invasion. This finding suggests IL-24

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Metastatic osteosarcoma has a poor prognosis with current treatments.
  • Effective treatments for osteosarcoma metastasis are urgently needed.
  • Interleukin-24 (IL-24) has shown potential in inhibiting cancer cell growth.

Purpose of the Study:

  • To investigate the potential of IL-24 as a therapeutic agent against osteosarcoma metastasis.
  • To determine the mechanism by which IL-24 affects osteosarcoma cell invasion and migration.

Main Methods:

  • Utilized spontaneously metastasizing human 143B osteosarcoma cells.
  • Assessed the effect of IL-24 on cell migration and invasion.
  • Investigated the involvement of the c-Jun N-terminal kinase (JNK)/c-Jun signaling pathway.
  • Measured the expression of matrix metalloproteinase (MMP)-2 and MMP-9.

Main Results:

  • IL-24 significantly inhibited migration and invasion in 143B osteosarcoma cells.
  • IL-24 suppressed the phosphorylation of JNK and c-Jun.
  • Downregulation of MMP-2 and MMP-9 expression was observed following IL-24 treatment.
  • These molecular changes contributed to the suppression of cell migration and invasion.

Conclusions:

  • IL-24 demonstrates potent metastasis-suppressive activity in highly metastatic osteosarcoma cells.
  • IL-24 acts via the JNK/c-Jun signaling pathway to reduce MMP-2 and MMP-9 levels.
  • IL-24 holds translational potential as a therapeutic agent for metastatic osteosarcoma.
Keywords:
interleukin-24invasionmetastasismigrationosteosarcoma

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