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Aspartic Acid Racemization with Correlation to Age: AForensic Perspective.

Qudsia Hassan1, Allah Rakha2, M Zahid Bashir3

  • 1Department of Forensic Medicine and Toxicology, Ziauddin University, Karachi.

Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
|June 11, 2017
PubMed
Summary

Aspartic acid racemization in human dentin offers a reliable method for age estimation in forensic analysis. This technique, utilizing High-Performance Liquid Chromatography, shows a strong correlation between chronological age and estimated age.

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Area of Science:

  • Forensic Science
  • Biochemistry
  • Anthropology

Background:

  • Accurate age estimation is crucial in forensic investigations.
  • Human dentin undergoes aspartic acid racemization, a process that changes with age.
  • Previous methods for age estimation have limitations.

Purpose of the Study:

  • To estimate age using aspartic acid racemization of human dentin.
  • To determine the margin of error between chronological and estimated age.
  • To evaluate the reliability of High-Performance Liquid Chromatography (HPLC) for this purpose.

Main Methods:

  • A cross-sectional comparative study was conducted.
  • Aspartic acid racemization (Land D ratio) was quantified in dentin samples using HPLC.
  • A regression equation was derived using 85 samples to estimate age based on the coefficient of racemization.

Main Results:

  • The regression equation demonstrated 74% accuracy in age estimation (coefficient of determination = 0.74).
  • A strong positive correlation (0.834) was found between chronological age and the coefficient of racemization.
  • Mean chronological and calibrated ages were statistically similar, with no significant differences based on gender or socioeconomic status.

Conclusions:

  • Aspartic acid racemization of human dentin is a reliable method for age estimation.
  • HPLC is a reproducible, cost-effective, and convenient technique for quantifying racemization.
  • Standardization of the procedure across laboratories is recommended for quality assurance.