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A Tuberculosis Molecular Bacterial Load Assay TB-MBLA
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A Tuberculosis Molecular Bacterial Load Assay TB-MBLA

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Mycobacterial Load Assay.

Stephen H Gillespie1, Wilber Sabiiti2, Katarina Oravcova2

  • 1School of Medicine, University of St Andrews, North Haugh, North Street, St Andrews, KY16 9AJ, UK. shg3@st-andrews.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2017
PubMed
Summary
This summary is machine-generated.

Tuberculosis treatment monitoring is challenging due to delayed results. The mycobacterial load assay (MBLA) offers a rapid molecular method to quantify viable Mycobacterium tuberculosis in patient samples.

Keywords:
Mycobacterial load assay (MBLA)Reverse transcriptase real-time quantitative polymerase chain reaction (RT-qPCR)Ribosomal RNA (rRNA)Sputum

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Tuberculosis (TB) treatment monitoring is hindered by assays yielding results long after sample collection.
  • Current methods lack the ability to quickly assess treatment efficacy or bacterial viability.
  • There is a critical need for rapid diagnostics in managing TB.

Purpose of the Study:

  • To introduce and evaluate the mycobacterial load assay (MBLA) as a novel tool for TB diagnostics.
  • To assess MBLA's capability in quantifying Mycobacterium tuberculosis (M.tb) load.
  • To determine MBLA's utility in assessing bacterial viability for treatment response.

Main Methods:

  • Development of a molecular assay for quantifying M.tb.
  • Validation of the assay using various sample types (human and other).
  • Assessment of M.tb viability through molecular detection.

Main Results:

  • The mycobacterial load assay (MBLA) provides a straightforward molecular test.
  • MBLA can quantify M. tuberculosis in biological samples.
  • The assay determines the viability of M. tuberculosis.

Conclusions:

  • MBLA offers a significant advancement in TB diagnostics by enabling rapid quantification and viability assessment.
  • This assay can potentially shorten the time to determine treatment response.
  • MBLA could improve patient management and outcomes for tuberculosis.