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Stainer and Scholte's pertussis medium with an alternative buffer
Abstract:
A comparative study of the Stainer and Scholte chemically defined growth medium (SS) for Bordetella pertussis, and a modification of it (MSS), was made. The Tris buffer which is reported to have adverse effects was replaced in MSS with sodium glycerophosphate. By using generation time as a measure of the growth promoting properties of the media, we found that the MSS was superior to SS for B. pertussis strain CN5612/67, which was used as a vaccine strain in Norway. Additionally by employing the 'dilution to extinction' method we showed that MSS supported B. pertussis growth from smaller inocula than the original SS medium. No significant differences in the quality of vaccines made from organisms grown in the two alternative media were observed in animal tests for potency and safety. The difference in buffers in SS and MSS had no influence on the formation of the agglutinogens, as tested by agglutination with specific factor sera. It was confirmed that liquid media gave a better total expression of the agglutinogens than solid media.
Insights
A modified Bordetella pertussis growth medium (MSS) using sodium glycerophosphate buffer is superior to the original Stainer and Scholte (SS) medium. MSS supports better bacterial growth and vaccine production quality, enhancing pertussis vaccine development.
Area of Science:
- Microbiology
- Vaccinology
- Biotechnology
Background:
- The Stainer and Scholte (SS) medium is a standard for Bordetella pertussis culture.
- Tris buffer in the SS medium may have adverse effects on bacterial growth.
Purpose of the Study:
- To compare the growth-promoting properties of the original SS medium and a modified version (MSS) for Bordetella pertussis.
- To evaluate the impact of medium modification on vaccine quality.
Main Methods:
- Comparative analysis of SS and MSS media using generation time and 'dilution to extinction' methods.
- Assessment of vaccine potency and safety in animal models.
- Agglutinogen expression analysis using specific factor sera.
Main Results:
- The MSS medium demonstrated superior growth promotion for Bordetella pertussis compared to the SS medium.
- MSS supported bacterial growth from smaller inocula than SS.
- No significant differences in vaccine potency or safety were observed between the two media.
- Buffer modification did not affect agglutinogen formation.
Conclusions:
- The modified Stainer and Scholte (MSS) medium is a more effective growth medium for Bordetella pertussis.
- MSS offers advantages for vaccine strain cultivation without compromising vaccine quality.
- Liquid media are preferable for optimal agglutinogen expression in Bordetella pertussis.