Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion

We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO₂ semiconductor nanoparticles as an efficient nanophotocatalyst for H₂ evolution. The system produces hydrogen at a turnover of about 240 μmol of H₂ (μmol protein)^-1 h^-1 and 17.74 mmol of H₂ (μmol protein)^-1 h^-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.