Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RAPT: Retrieval-Augmented Visual Prompting with Text-Guidance for Pathological Image Classification.

IEEE journal of biomedical and health informatics·2026
Same author

Nitrogen-Vacancy Centers in Fluorescent Nanodiamonds: Emerging Applications from Healthcare Diagnostics to Semiconductor Metrology.

Accounts of chemical research·2026
Same author

A text-guided Brownian bridge diffusion model for unified multiphase contrast-enhanced CT synthesis.

Biomedical physics & engineering express·2026
Same author

Supramolecular self-assembly mechanisms, interfacial behavior, multimodal antimicrobial and anti-adhesion activities of the novel Tilapia piscidin-3 (TP3) peptide hydrogel.

Colloids and surfaces. B, Biointerfaces·2026
Same author

The Crucial Role of LTTR_0390 in <i>Burkholderia gladioli</i> BBB-01 in Orchestrating Antibiotic Production, Quorum-Sensing Responses, and Pathogenicity on Mushrooms.

Journal of agricultural and food chemistry·2026
Same author

Profiling High-Abundance Serum Proteins in the Corona of Nanodiamonds Using Mass Spectrometry.

Langmuir : the ACS journal of surfaces and colloids·2026

Related Experiment Video

Updated: Feb 28, 2026

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
09:15

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Published on: August 14, 2018

11.1K

Nanodiamonds as Nucleating Agents for Protein Crystallization.

Yen-Wei Chen1, Chien-Hsun Lee1, Yung-Lin Wang2

  • 1Institute of Atomic and Molecular Sciences, Academia Sinica , Taipei 106, Taiwan.

Langmuir : the ACS Journal of Surfaces and Colloids
|June 13, 2017
PubMed
Summary

Surface-modified nanodiamonds (NDs) effectively promote protein crystallization. This approach enhances crystallization efficiency for various proteins, offering a versatile tool for biochemical research.

More Related Videos

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
13:02

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Published on: December 28, 2019

8.0K
Optimization of Crystal Growth for Neutron Macromolecular Crystallography
12:29

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Published on: March 13, 2021

6.0K

Related Experiment Videos

Last Updated: Feb 28, 2026

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
09:15

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Published on: August 14, 2018

11.1K
Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
13:02

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Published on: December 28, 2019

8.0K
Optimization of Crystal Growth for Neutron Macromolecular Crystallography
12:29

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Published on: March 13, 2021

6.0K

Area of Science:

  • Materials Science
  • Biochemistry
  • Nanotechnology

Background:

  • Nanodiamonds (NDs) are carbon-based nanomaterials with promising biological applications.
  • Facilitating protein crystal nucleation in aqueous solutions is a key challenge in structural biology.

Purpose of the Study:

  • To investigate the utility of surface-modified nanodiamonds as heterogeneous nucleating agents for protein crystallization.
  • To evaluate the impact of nanodiamond size and form on crystallization efficiency.

Main Methods:

  • Surface oxidation of nanodiamonds (30 and 100 nm) followed by acid treatment.
  • Utilizing nanodiamond aggregates and films as nucleation sites for protein crystallization.
  • Testing crystallization efficiency with model proteins: lysozyme, ribonuclease A, proteinase K, and catalase.

Main Results:

  • Surface-modified nanodiamonds significantly increased crystallization efficiency for all tested proteins.
  • 30 nm nanodiamond films (∼2 mm²) effectively induced lysozyme crystallization at 5 mg/mL.
  • Combining nanodiamonds with preformed protein clusters further improved crystallization efficiency for ribonuclease A.

Conclusions:

  • Surface-modified nanodiamonds serve as efficient heterogeneous nucleating agents for protein crystallization.
  • The method is versatile, compatible with existing techniques, and applicable to diverse protein samples.
  • This approach simplifies and enhances protein crystallization, aiding structural studies.