Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.6K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design, Synthesis, and Evaluation of Bibenzyl Analogues against Hepatocellular Carcinoma by Targeting Pyruvate Carboxylase.

Journal of medicinal chemistry·2026
Same author

The signaling and regulatory functions of dying cells and cell corpses.

Communications biology·2026
Same author

Knocking down FAM110A suppresses colon adenocarcinoma progression by inhibiting the Nrf2/HO-1 axis to induce ferroptosis.

World journal of surgical oncology·2026
Same author

In vivo membrane engineering traps Gd-based MRI contrast agents for detecting microhepatocellular carcinoma.

Science advances·2026
Same author

Teaching Neuraxial Ultrasonography with a Virtual Reality Simulator of Spine: A Randomized Controlled Study.

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih·2026
Same author

Janus polymeric discs by seeded swelling emulsion polymerization.

Nanoscale·2026
Same journal

Aptamer-CRISPR Glucose Transducer for point-of-care IgE detection.

Talanta·2026
Same journal

Dual-channel fluorescent probes enable synchronous tracking of peroxynitrite and cysteine in mitochondrial redox dynamics.

Talanta·2026
Same journal

A versatile graphene-like film as a chemo-resistive platform for selective ammonia gas sensing.

Talanta·2026
Same journal

Enhanced determination of 85 mycotoxins in challenging root and rhizome herbal medicines using online segmented multi-dimensional liquid chromatography-tandem mass spectrometry.

Talanta·2026
Same journal

Tailoring oxygen vacancy of WO<sub>3</sub> nanoparticles for high-performance gas sensing: room-temperature NO<sub>2</sub> and low-temperature triethylamine detection.

Talanta·2026
Same journal

Mixed potential acetone sensor based on LaBaCo<sub>2-x</sub>Fe<sub>x</sub>O<sub>5±δ</sub> (x=0, 0.05 and 0.2) sensing electrode and yttria-stabilized zirconia for non-invasive diagnosis of diabetes.

Talanta·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.5K

Nucleobase chemosensor based on carbon nanodots.

Yu Hou1, Xue Liu1, Xiuping Tang1

  • 1Liaoning Province Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, College of Chemistry, Liaoning University, Shenyang 110036, PR China.

Talanta
|June 13, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a new fluorescence method using carbon nanodots (CDs) made from nucleobases for sensitive detection. These CD chemosensors can accurately quantify specific nucleobases in solutions, showing great potential for biological sensing.

Keywords:
Carbon nanodotsChemosensorFluorescenceNucleobase detection

More Related Videos

Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.6K
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.6K

Related Experiment Videos

Last Updated: Feb 28, 2026

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.5K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.6K
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.6K

Area of Science:

  • Biotechnology
  • Materials Science
  • Analytical Chemistry

Background:

  • Nucleobase detection is crucial for biological and medical diagnostics.
  • Existing methods for nucleobase detection often lack sensitivity or require complex procedures.
  • Carbon nanodots (CDs) offer unique optical properties for developing novel biosensors.

Purpose of the Study:

  • To develop a facile and sensitive fluorescence protocol for nucleobase detection using carbon nanodot (CD) chemosensors.
  • To synthesize and characterize four types of nucleobase-based CDs (adenine, guanine, thymine, cytosine).
  • To investigate the sensing mechanism and quantitative assay capabilities of these CD chemosensors.

Main Methods:

  • Synthesis of fluorescent CDs using nucleobases (adenine, guanine, thymine, cytosine) as carbon sources via hydrothermal strategy.
  • Characterization of CD properties, including quantum yield.
  • Investigation of complementary base pairing for selective nucleobase recognition.
  • Quantitative analysis of nucleobases through fluorescence quenching and calibration curves.
  • Evaluation of sensor performance in biological media like fetal calf serum.

Main Results:

  • Four types of nucleobase-based CDs (A-CDs, G-CDs, T-CDs, C-CDs) were successfully synthesized with varying quantum yields.
  • CDs demonstrated selective recognition of complementary nucleobases via fluorescence quenching.
  • Quantitative detection of thymine using A-CDs was achieved in the range of 2-20 mM with a detection limit of 0.053 mM.
  • A dynamic quenching mechanism was identified for thymine detection.
  • The sensing system exhibited high accuracy and credibility, with low relative standard deviation (<4.0%) in fetal calf serum.

Conclusions:

  • A novel and sensitive fluorescence sensing platform for nucleobase detection based on nucleobase-derived CDs was established.
  • The developed CD chemosensors exhibit excellent sensitivity, good biocompatibility, and a simple construction method.
  • This sensing platform holds significant potential for various biological sensing applications.