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Published on: November 11, 2015
Mycoplasma fermentans deacetylase promotes mammalian cell stress tolerance
Qingzhou Cheng1, Lijuan Wu1, Rongfu Tu2
1College of Health Sciences and Nursing, Wuhan Polytechnic University, Wuhan, Hubei, China.
Abstract:
Mycoplasma fermentans is a pathogenic bacterium that infects humans and has potential pathogenic roles in respiratory, genital and rheumatoid diseases. NAD+-dependent deacetylase is involved in a wide range of pathophysiological processes and our studies have demonstrated that expression of mycoplasmal deacetylase in mammalian cells inhibits proliferation but promotes anti-starvation stress tolerance. Furthermore, mycoplasmal deacetylase is involved in cellular anti-oxidation, which correlates with changes in the proapoptotic proteins BIK, p21 and BIM. Mycoplasmal deacetylase binds to and deacetylates the FOXO3 protein, similar with mammalian SIRT2, and affects expression of the FOXO3 target gene BIM, resulting in inhibition of cell proliferation. Mycoplasmal deacetylase also alters the performance of cells under drug stress. This study expands our understanding of the potential molecular and cellular mechanisms of interaction between mycoplasmas and mammalian cells.
Insights
Mycoplasma fermentans deacetylase inhibits mammalian cell proliferation and enhances stress tolerance. This bacterial enzyme targets FOXO3, impacting cell survival and drug responses.
Area of Science:
- Microbiology
- Cell Biology
- Molecular Biology
Background:
- Mycoplasma fermentans is a pathogen linked to various human diseases.
- NAD+-dependent deacetylases play crucial roles in cellular processes.
- Understanding mycoplasma-host interactions is vital for disease management.
Purpose of the Study:
- To investigate the function of Mycoplasma fermentans deacetylase in mammalian cells.
- To elucidate the molecular mechanisms underlying its effects on cell proliferation and stress tolerance.
- To explore its role in cellular anti-oxidation and drug response.
Main Methods:
- Expression of mycoplasma deacetylase in mammalian cells.
- Analysis of cell proliferation, stress tolerance, and anti-oxidation.
- Western blotting to detect changes in proapoptotic proteins (BIK, p21, BIM).
- Investigation of FOXO3 protein binding and deacetylation.
Main Results:
- Mycoplasma deacetylase inhibited cell proliferation but enhanced anti-starvation stress tolerance.
- The enzyme modulated cellular anti-oxidation, affecting BIK, p21, and BIM levels.
- Mycoplasma deacetylase deacetylated FOXO3, impacting BIM expression and cell proliferation.
- Altered cellular performance under drug stress was observed.
Conclusions:
- Mycoplasma fermentans deacetylase influences mammalian cell behavior, including proliferation and stress responses.
- The interaction involves deacetylation of FOXO3, highlighting a conserved mechanism with mammalian SIRT2.
- This study provides insights into the molecular basis of mycoplasma pathogenesis.
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