Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct incorporation of microalgal biomass into polymer composites and foams: a chemical engineering perspective toward sustainable bioplastics.

Bioresource technology·2026
Same author

Thermotolerant Chlorella vulgaris sustains flue gas carbon capture and nitrogen oxide and sulfur oxide mitigation through thylakoid remodelling, proteostasis, and antioxidant defense.

Bioresource technology·2026
Same author

Phytohormone-Assisted Bioprocess Engineering for Enhanced Omega Fatty Acid Production in Marine Thraustochytrids Under Cold Stress Conditions.

Marine biotechnology (New York, N.Y.)·2026
Same author

Copper stress responses in Scenedesmus obliquus-Bacillus subtilis Consortia: Machine Learning-Based prediction of copper removal.

Bioresource technology·2026
Same author

Corrigendum to "Utilization of current pyrolysis technology to convert biomass and manure waste into biochar for soil remediation: A review" [Sci. Total Environ., 864 (2023), 160990].

The Science of the total environment·2026
Same author

Aerodynamic performance optimization of the archimedes spiral wind turbine: combined experimental and CFD analysis of step ratio and blade number effects.

Scientific reports·2026

Related Experiment Video

Updated: Feb 28, 2026

Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

61.7K

Proteins recovery from wet microalgae using liquid biphasic flotation (LBF).

Win Nee Phong1, Pau Loke Show2, Wei Heng Teh3

  • 1Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Bioresource Technology
|June 13, 2017
PubMed
Summary

This study optimized alcohol/salt liquid biphasic flotation (LBF) with ultrasonication for microalgal protein extraction from Chlorella sorokiniana. Optimized conditions achieved 88.86% protein recovery after three recycling runs, reducing alcohol and salt usage.

Keywords:
Liquid biphasic flotation (LBF)MicroalgaeProtein recoveryUltrasonication

More Related Videos

Coupling Carbon Capture from a Power Plant with Semi-automated Open Raceway Ponds for Microalgae Cultivation
08:17

Coupling Carbon Capture from a Power Plant with Semi-automated Open Raceway Ponds for Microalgae Cultivation

Published on: August 14, 2020

6.0K
Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Published on: January 7, 2019

22.6K

Related Experiment Videos

Last Updated: Feb 28, 2026

Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

61.7K
Coupling Carbon Capture from a Power Plant with Semi-automated Open Raceway Ponds for Microalgae Cultivation
08:17

Coupling Carbon Capture from a Power Plant with Semi-automated Open Raceway Ponds for Microalgae Cultivation

Published on: August 14, 2020

6.0K
Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Published on: January 7, 2019

22.6K

Area of Science:

  • Biotechnology
  • Protein Extraction
  • Microalgae

Background:

  • Microalgal biomass is a rich source of protein.
  • Efficient protein extraction methods are crucial for valorizing microalgae.
  • Current methods often require harsh conditions or extensive processing.

Purpose of the Study:

  • To develop and optimize an alcohol/salt liquid biphasic flotation (LBF) process for microalgal protein extraction.
  • To investigate the impact of various parameters on protein recovery efficiency.
  • To assess the feasibility of recycling phase components to improve process sustainability.

Main Methods:

  • Utilized ultrasonication for cell disruption of Chlorella sorokiniana.
  • Employed alcohol/salt liquid biphasic flotation (LBF) for protein separation.
  • Systematically varied parameters including feedstock concentration, salt type and concentration, alcohol type and concentration, and flotation time.
  • Implemented a phase component recycling strategy.

Main Results:

  • Optimized conditions yielded a high protein recovery of 88.86% in the top phase after three recycling runs.
  • Key optimal parameters included 250g/L ammonium sulfate, 60% (v/v) 2-propanol, 20g/L crude biomass load, and 10 minutes flotation time.
  • Recycling of the alcohol phase demonstrated increasing performance over consecutive runs, reducing solvent requirements.

Conclusions:

  • Alcohol/salt LBF is an effective method for extracting protein from wet microalgal biomass.
  • Ultrasonication enhances cell disruption, improving protein accessibility.
  • Phase component recycling significantly enhances the economic and environmental viability of the LBF process for microalgal protein recovery.