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Published on: May 13, 2016
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Progress and biotechnological prospects in fish transgenesis
Fernanda M P Tonelli1, Samyra M S N Lacerda2, Flávia C P Tonelli3
1Laboratório de Sinalização Celular e Nanobiotecnologia, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Instituto Nanocell, Divinópolis, MG, Brazil.
Biotechnology Advances
|June 13, 2017
Summary
Transgenic animal development, especially in fish, has advanced significantly since 1984. Modern techniques like stem cell modification revolutionize genetic engineering for aquaculture applications.
Area of Science:
- Animal biotechnology
- Genetic engineering
- Aquaculture science
Background:
- Transgenesis history includes cellular transdifferentiation and recombinant DNA technologies.
- The first transgenic fish was created in 1984, spurring advancements in gene transfer methods.
- Genetic engineering has evolved, enabling more efficient and accurate generation of transgenic organisms.
Purpose of the Study:
- To review the history of animal transgenesis, focusing on fish.
- To highlight key developments in genetic engineering relevant to transgenesis.
- To discuss the biotechnological prospects and applications of fish transgenesis in aquaculture.
Main Methods:
- Review of historical milestones in transgenesis.
- Emphasis on techniques like microinjection, electroporation, lipofection, viral vectors, and gene targeting.
- Focus on spermatogonial stem cell modification and transplantation for transgenic fish generation.
Main Results:
- Technological advancements have led to rapid, accurate, and efficient generation of transgenic animals.
- Spermatogonial stem cell techniques show revolutionary potential for transgenic fish.
- Transgenic fish are emerging in the market, demonstrating commercial viability.
Conclusions:
- Fish transgenesis has evolved significantly, with stem cell technologies offering new possibilities.
- Continued biotechnological advancements are crucial for improving economically important fish species.
- Transgenic fish hold substantial promise for the aquaculture industry's future growth and sustainability.
