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CRISPR/Cas9: From Genome Engineering to Cancer Drug Discovery

Ji Luo1

  • 1Laboratory of Cancer Biology and Genomics, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20814, USA.

Trends in Cancer
|June 13, 2017
PubMed

Insights

New technologies like CRISPR gene editing are revolutionizing cancer research. This review explores how CRISPR advances functional genomics and aids in discovering novel cancer drug targets.

Area of Science:

  • Genomics
  • Molecular Biology
  • Cancer Research

Background:

  • Technological advancements drive translational research in oncology.
  • Microarrays, next-generation sequencing, proteomics, and RNA interference (RNAi) have improved cancer mechanism understanding and drug target discovery.
  • The bacterial clustered regularly interspaced palindromic repeat (CRISPR) system offers new possibilities for functional genomics.

Purpose of the Study:

  • To review the utility of CRISPR technology for cancer drug target discovery.
  • To highlight CRISPR's role in advancing functional genomics in cancer research.

Main Methods:

  • Review of existing literature on CRISPR applications in cancer research.
  • Focus on CRISPR's adaptation for mammalian genome engineering.
  • Analysis of CRISPR's contribution to identifying potential cancer drug targets.

Main Results:

  • CRISPR enables precise genome engineering for functional genomics studies.
  • CRISPR facilitates the identification and validation of novel cancer drug targets.
  • The technology accelerates the understanding of cancer biology.

Conclusions:

  • CRISPR technology is a powerful tool for cancer drug target discovery.
  • Its application in functional genomics is transforming cancer research avenues.
  • Further exploration of CRISPR's potential in oncology is warranted.

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