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Updated: Feb 28, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
Published on: April 21, 2022
Visualizing adenosine-to-inosine RNA editing in single mammalian cells
Ian A Mellis1,2, Rohit Gupte1, Arjun Raj1,2,3
1Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Abstract:
Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about the biology of this conversion remain unknown because of a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine-edited transcripts in situ. We found that editing of the GRIA2, EIF2AK2, and NUP43 transcripts is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells, while GRIA2 editing levels vary.
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