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Updated: Feb 28, 2026
Bioluminescent Bacterial Imaging In Vivo
Published on: November 4, 2012
Bioluminescence Sensing of γ-Glutamyltranspeptidase Activity In Vitro and In Vivo
Zijuan Hai1, Jingjing Wu1, Lin Wang2
1CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , Hefei, Anhui 230026, China.
Abstract:
γ-Glutamyltranspeptidase (GGT) is an important tumor biomarker but using a bioluminescence (BL) probe to real time monitor its activity has not been reported. Herein, we rationally designed two GGT-cleavable BL probes Glu-AmLH2 (1) and Glu-p-aminobenzyloxycarbonyl-AmLH2 (2), and successfully applied them for sensing GGT activity with high sensitivity and excellent selectivity both in vitro and in vivo. The results indicated that, although 2 had lower background BL signal than 1, GGT had higher catalytic efficiency for 1 than 2, and 1 was superior to 2 for sensing GGT activity in living cells and tumors. We envision that our probe 1 could be widely applied for the diagnosis of important GGT-related diseases in animal models in the near future.
