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Assembly and Characterization of Polyelectrolyte Complex Micelles
Published on: March 2, 2020
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Ternary polysaccharide complexes: Colloidal drug delivery systems stabilized in physiological media
Danjun Wu1, Agathe Ensinas2, Bernard Verrier2
1Ingénierie des Matériaux Polymères, UMR CNRS 5223, Université Claude Bernard Lyon 1, 15 Bd. André Latarjet, 69622 Villeurbanne Cedex, France.
Carbohydrate Polymers
|June 14, 2017
Summary
New ternary complexes of chitosan and hyaluronan (HYA) offer enhanced colloidal stability for drug delivery. These stabilized nanoparticles effectively delivered tenofovir, showing superior HIV-1 inhibition in vitro.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery Systems
Background:
- Developing stable colloidal systems for drug delivery is crucial.
- Chitosan-hyaluronan (HYA) polyelectrolyte complexes (PECs) require stabilization strategies for physiological conditions.
- Ternary complex formation offers a novel approach to enhance PEC stability.
Purpose of the Study:
- To design stable chitosan-hyaluronan (HYA) polyelectrolyte complexes (PECs) using a ternary complex strategy.
- To investigate the influence of polymer ratios and functionalization on colloidal stability.
- To evaluate the efficacy of drug-loaded ternary complexes for HIV-1 inhibition.
Main Methods:
- Formation of binary PECs between chitosan and strong polyacids (dextran sulphate or heparin).
- Functionalization of binary PECs with hyaluronan (HYA) to create ternary complexes.
- Loading of tenofovir (TF) into stable ternary complexes and surface functionalization with IgAs.
- In vitro evaluation of HIV-1 inhibition in PBMCs using cationic TF-loaded ternary complexes.
Main Results:
- A high ratio of chitosan to strong polyacid degree of polymerization was key for stabilizing colloids.
- Ternary complexes could be engineered to yield positive or negative particles based on HYA stoichiometry.
- Tenofovir (TF)-loaded ternary complexes demonstrated superior in vitro inhibition of HIV-1 infection in PBMCs compared to the free drug.
- Surface functionalization with IgAs was achieved for targeted delivery.
Conclusions:
- Ternary complexation is an effective strategy for stabilizing chitosan-hyaluronan (HYA) polyelectrolyte complexes (PECs) at physiological conditions.
- The developed ternary complexes show promise as advanced drug delivery systems for antiretroviral therapy.
- These novel nanocarriers exhibit enhanced efficacy in inhibiting HIV-1 infection in vitro.
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