Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Higher-order Actin Filaments01:11

Formation of Higher-order Actin Filaments

3.7K
The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
The high-order actin...
3.7K
Drugs Used in Upper Respiratory Disorders: Overview01:16

Drugs Used in Upper Respiratory Disorders: Overview

805
Upper respiratory tract disorders, including viral infections and allergic rhinitis, cause significant discomfort and disrupt daily life. Managing these conditions involves a variety of drugs, such as antihistamines, intranasal steroids, decongestants, antitussives, expectorants, and mucolytics. Specific examples of drugs in each category are provided.
Antihistamines (e.g., Benadryl) block histamines from binding. Histamines are chemicals released during an allergic reaction in the body. As a...
805
Generation of Straight or Branched Actin Filaments01:14

Generation of Straight or Branched Actin Filaments

3.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
3.9K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

28.0K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
28.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glycolysis-enhancing α<sub>1</sub>-adrenergic antagonists have therapeutic potential in Alzheimer's disease.

Alzheimer's research & therapy·2026
Same author

<i>CFTR</i> gene delivery to human airway epithelia using parainfluenza virus 5 amplifying virus-like particles.

Molecular therapy. Nucleic acids·2026
Same author

Case study of low-cost energy reductions in shared research laboratories at a U.S. public university.

Frontiers in sustainability·2026
Same author

Dietary fiber selectively regulates intestinal persistence of probiotic bifidobacteria.

bioRxiv : the preprint server for biology·2026
Same author

What the nose knows of cystic fibrosis microbes and hypertonic saline.

Cell host & microbe·2026
Same author

Refueling T cell memory in cystic fibrosis.

American journal of respiratory cell and molecular biology·2026

Related Experiment Video

Updated: Feb 28, 2026

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
09:24

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Published on: June 14, 2016

27.2K

Gel-forming mucins form distinct morphologic structures in airways.

Lynda S Ostedgaard1, Thomas O Moninger1, James D McMenimen1

  • 1Department of Internal Medicine, Pappajohn Biomedical Institute, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242.

Proceedings of the National Academy of Sciences of the United States of America
|June 14, 2017
PubMed
Summary

Airway mucus mucins MUC5B and MUC5AC have distinct structures and interactions. Understanding these differences in cystic fibrosis (CF) airways is key to improving mucociliary transport and disease management.

Keywords:
COPDasthmacystic fibrosislungmucus

More Related Videos

Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer
08:47

Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer

Published on: April 21, 2022

4.0K
Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
08:51

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Published on: June 20, 2025

719

Related Experiment Videos

Last Updated: Feb 28, 2026

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
09:24

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Published on: June 14, 2016

27.2K
Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer
08:47

Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer

Published on: April 21, 2022

4.0K
Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
08:51

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Published on: June 20, 2025

719

Area of Science:

  • Pulmonary Medicine
  • Biochemistry
  • Cell Biology

Background:

  • Gel-forming mucins are key to airway clearance via mucociliary transport.
  • Altered mucus properties in cystic fibrosis (CF) impair this transport.
  • The specific roles and structures of MUC5B and MUC5AC mucins in healthy and CF airways are not fully understood.

Purpose of the Study:

  • To investigate the distinct morphologies and associations of MUC5B and MUC5AC mucins.
  • To compare mucin structures in healthy porcine airways versus early-stage CF porcine airways.
  • To elucidate the contributions of MUC5B and MUC5AC to mucociliary transport in health and disease.

Main Methods:

  • Examination of freshly excised airways from newborn non-CF and CF pigs.
  • Microscopic analysis of mucin structures and their origins (submucosal glands vs. goblet cells).
  • Comparative analysis of mucin distribution and morphology in non-CF and CF airways.

Main Results:

  • Porcine submucosal glands secrete MUC5B; goblet cells secrete MUC5AC and some MUC5B.
  • MUC5B forms filamentous strands from submucosal glands; MUC5AC forms threads/sheets from goblet cells.
  • MUC5AC often coats MUC5B strands, with increased MUC5B in CF gland ducts and MUC5AC sheet accumulation in CF airways.

Conclusions:

  • MUC5B and MUC5AC exhibit distinct morphologies and interactions within the airway mucus.
  • These mucins likely play different roles in mucociliary transport.
  • The observed differences provide a framework for understanding mucin abnormalities in CF and other airway diseases.