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Updated: Feb 28, 2026
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
Published on: July 24, 2017
Zymography Methods to Simultaneously Analyze Superoxide Dismutase and Catalase Activities: Novel Application for
Esther Gamero-Sandemetrio1, Rocío Gómez-Pastor1,2, Emilia Matallana3,4
1Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Avda Agustín Escardino, 7, Paterna, 46980, Valencia, Spain.
Abstract:
We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H2O2 allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.
