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Updated: Feb 28, 2026

An Easy Method for Plant Polysome Profiling
Published on: August 28, 2016
Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana
Cécile Lecampion1,2,3, Elodie Lanet1,2,3, Christophe Robaglia4,5,6
1Laboratoire de Génétique et Biophysique des Plantes, Aix Marseille Univ, Marseille, F-13009, France.
Abstract:
Argonaute (AGO) proteins play a key role in RNA silencing mechanisms. RNA silencing affects both RNA degradation and translation. The characterization of translation-associated RNA silencing mechanisms and components often requires polysome isolation and analysis. In this chapter, we describe the identification of AGO1 association with polysomes through polysome fractionation on sucrose gradient, preparation of proteins by filtration and concentration, and immunoblotting.
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