ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation
Meizhen Wang1, Yuxing Deng1, Fenjuan Shao2
1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, No.151, Malianwa North Road, Haidian District, Beijing, 100193, China.
Abstract:
Small RNA-mediated gene silencing is a vital regulatory mechanism in eukaryotes that requires ARGONAUTE (AGO) proteins. Salvia miltiorrhiza is a well-known traditional Chinese medicinal plant. Therefore, it is important to characterize S. miltiorrhiza AGO family genes as they may be involved in multiple metabolic pathways. This chapter introduces the detailed protocol for SmAGO gene prediction and molecular cloning. In addition, an Agrobacterium-mediated genetic transformation method for S. miltiorrhiza is presented. These methodologies can be used to functionally study SmAGO genes as well as other genes of interest in S. miltiorrhiza.
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