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Cloning and Characterization of Argonaute Genes in Tomato
Zhiqiang Xian1, Fang Yan1, Zhengguo Li2
1School of Life Sciences, Chongqing University, No 702 Room, Campus D, Chongqing, 401331, China.
Abstract:
Argonaute (AGO) proteins are core elements in plant posttranscriptional RNA silencing pathways. The identification and functional characterization of tomato (Solanum lycopersicum) AGOs will help to better understand RNA silencing-based posttranscriptional regulation in fleshy fruits. Here we describe how to identify and clone SlAGO genes, as well as the methodology for their functional characterization.
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