Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrating diversity, equity, and inclusion in generative AI applications for healthcare education: a scoping review.

International journal of medical informatics·2026

Same author

Enhancing Next-Generation Sequencing Sensitivity with High-Recovery Adapter Ligation and Cas9-Mediated Dimer Elimination.

Clinical chemistry·2026

Same author

The Role of Adjuvant Chemotherapy in High-Risk Stage II Colon Cancer with Microsatellite Instability-High or DNA Mismatch Repair Deficiencies: A Multicenter Pooled Analysis (KCSG-CO24-03).

Cancer research and treatment·2026

Same author

Metformin Promotes Acquisition of Immune Tolerance in Allergen-Specific Immunotherapy.

Allergy, asthma & immunology research·2026

Same author

Preliminary normative study of Cognosis: A digital cognitive assessment battery for Korean elders.

Digital health·2026

Same author

Intravenous immunotherapy with Der p 1-containing nanoparticles alleviates the allergic responses in a murine allergic rhinitis model.

Human vaccines & immunotherapeutics·2026

Search research articles

Related Experiment Video

Updated: Feb 28, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

PEG3 control on the mammalian MSL complex.

An Ye¹, Hana Kim¹, Joomyeong Kim¹

¹Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, United States of America.

|June 14, 2017

Summary

This summary is machine-generated.

Paternally expressed gene 3 (Peg3) acts as a transcriptional repressor, controlling MSL complex components and H4K16ac levels. Its absence impacts gene expression and histone modification, including Hox clusters, in mammalian development.

More Related Videos

Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins

Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins

Published on: November 30, 2021

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Related Experiment Videos

Last Updated: Feb 28, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins

Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins

Published on: November 30, 2021

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Area of Science:

Epigenetics and Gene Regulation
Molecular Biology
Mammalian Development

Background:

Paternally expressed gene 3 (Peg3) is a DNA-binding protein functioning as a transcriptional repressor.
Recent findings indicate PEG3 interacts with Msl1 and Msl3, key components of the male-specific lethal (MSL) complex.
The MSL complex is known for its role in histone modification, specifically H4K16ac.

Purpose of the Study:

To investigate the role of Peg3 in regulating downstream genes via H4K16ac, a histone modification mediated by the MSL complex.
To confirm Peg3's function as a transcriptional repressor for MSL components during mammalian development.

Main Methods:

Analysis of gene expression and H4K16ac levels in MEF cells lacking PEG3.
Genome-wide analyses to identify affected genes and their promoter regions.
Assessment of changes in Hox clusters related to H4K16ac levels.

Main Results:

Complete removal of PEG3 led to increased Msl1 and Msl3 expression and elevated global H4K16ac levels.
Approximately 10% of the mouse gene catalog showed altered H4K16ac levels in promoter regions of PEG3-deficient MEF cells.
A subset of these affected genes exhibited increased expression, and three Hox clusters showed changes in H4K16ac.

Conclusions:

Peg3 functions as a transcriptional repressor of MSL components in mammals.
Peg3 influences global H4K16ac levels and regulates a significant portion of the gene catalog.
Peg3 and the MSL complex may play a role in the regulation of Hox clusters.