Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.5K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.5K
Chromatin Packaging02:21

Chromatin Packaging

22.6K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
22.6K
Chromatin Packaging01:32

Chromatin Packaging

19.9K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
19.9K
Chromatin Packaging02:21

Chromatin Packaging

10.0K
10.0K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

7.8K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.8K
Nucleosome Remodeling02:54

Nucleosome Remodeling

11.4K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A simple and versatile fluorescence-based method to enhance prime editing in human pluripotent stem cells.

BMC methods·2026
Same author

Beyond The Nucleus: Translating Engineered Protein Localization To Chromatin Modifying Enzymes.

Current opinion in biomedical engineering·2026
Same author

Special Issue on Mammalian Cell Synthetic Biology.

ACS synthetic biology·2026
Same author

Early Epigenetic and Metabolic Responses to the Adipocyte Secretome Reveal Stress-Adaptive States in Triple-Negative Breast Cancer.

bioRxiv : the preprint server for biology·2026
Same author

Ectopic expression to synthetic design: Deriving engineering principles of lncRNA-mediated epigenetic regulation.

Cell systems·2025
Same author

Decoding Gene Responsiveness to Synthetic Chromatin Reader-Actuators with Multi-Modal Epigenomic Profiling.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Feb 28, 2026

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

2.3K

Molecular structures guide the engineering of chromatin.

Stefan J Tekel1, Karmella A Haynes1

  • 1School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA.

Nucleic Acids Research
|June 14, 2017
PubMed
Summary

Understanding chromatin protein structure and function is key to engineering synthetic epigenetic systems. This research explores design rules for manipulating chromatin dynamics in medicine and agriculture.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Synthetic Biology

Background:

  • Chromatin organizes and regulates genetic information via DNA, RNA, and proteins in eukaryotic nuclei.
  • Chromatin proteins are crucial for DNA packing, nuclear organization, and gene expression control.
  • Manipulating chromatin dynamics offers potential applications in medicine and agriculture.

Purpose of the Study:

  • To identify design rules for the chromatin system by examining the relationship between physical structure and function of chromatin proteins.
  • To inform the development of synthetic systems by understanding intrinsic chromatin protein properties.
  • To advance synthetic epigenetics through engineering chromatin.

Main Methods:

  • Review of key research on chromatin protein properties.

More Related Videos

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
09:13

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

4.5K
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

8.9K

Related Experiment Videos

Last Updated: Feb 28, 2026

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

2.3K
Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
09:13

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

4.5K
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

8.9K

  • Analysis of structure-function relationships in chromatin proteins.
  • Exploration of portable chromatin-derived peptide motifs.

    • Main Results:

    • Intrinsic properties of chromatin proteins provide insights into design rules for synthetic systems.
    • Chromatin-derived peptide motifs demonstrate portability and customizability for altered function.
    • A workflow for fusion protein design and best practices for chromatin engineering are presented.

    Conclusions:

    • Understanding chromatin protein physical structure and function is essential for designing synthetic epigenetic systems.
    • Customizable peptide motifs offer a pathway for engineering chromatin.
    • This work provides a framework to advance synthetic epigenetics research and applications.