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Published on: April 15, 2016
Effect of NDC80 in human hepatocellular carcinoma
Lin-Ling Ju1, Lin Chen1, Jun-Hong Li1
1Lin-Ling Ju, Lin Chen, Yi-Fan Wang, Ru-Jian Lu, Zhao-Lian Bian, Jian-Guo Shao, Nantong Institute of Liver Diseases, Nantong Third People's Hospital, Nantong University, Nantong 226006, Jiangsu Province, China.
Aim:
To investigate the role of nuclear division cycle (NDC)80 in human hepatocellular carcinogenesis.
Methods:
NDC80 gene expression was analyzed by real-time reverse transcription polymerase chain reaction in 47 paired hepatocellular carcinoma (HCC) and adjacent tissues. The HCC cell line SMMC-7721 was transfected with lentivirus to silence endogenous NDC80 gene expression, which was confirmed by real-time polymerase chain reaction and western blotting. The effects of NDC80 silencing on SMMC-7721 cell proliferation were evaluated by Cellomics ArrayScan VTI imaging. Cell cycle analysis and apoptosis were detected with flow cytometry. Colony formation was assessed by fluorescence microscopy.
Results:
NDC80 expression levels in HCC tissues were significantly higher than those in the adjacent tissues. Functional studies demonstrated that NDC80 silencing significantly reduced SMMC-7721 cell proliferation and colony formation. Knockdown of NDC80 resulted in increased apoptosis and cell cycle arrest at S-phase. NDC80 contributed to HCC progression by reducing apoptosis and overcoming cell cycle arrest.
Conclusion:
Elevated expression of NDC80 may play a role in promoting the development of HCC.
