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Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
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Cell-laden microgel prepared using a biocompatible aqueous two-phase strategy.

Yang Liu1, Natalia Oshima Nambu2, Masahito Taya3

  • 1Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka, 560-8531, Japan. liuyang@cheng.es.osaka-u.ac.jp.

Biomedical Microdevices
|June 15, 2017
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Summary

This study introduces an aqueous two-phase system for microgel production, enhancing cell viability and overcoming limitations of oil-based methods. This novel approach improves microgel fabrication efficiency for biomedical applications.

Keywords:
Aqueous two-phase systemCell adhesionCell encapsulationMicrofluidicsMicrogel

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Area of Science:

  • Biomaterials Science
  • Microfluidics
  • Cell Encapsulation

Background:

  • Microfluidic production of cell-laden microgels commonly uses oil-water systems.
  • Oil-based systems present challenges in reagent distribution and microgel extraction, limiting efficiency.
  • Existing methods struggle with reagent dispersion and post-production processing.

Purpose of the Study:

  • To develop a novel microfluidic method for cell-laden microgel production using an aqueous two-phase system (ATPS).
  • To overcome the limitations of oil-water systems in microgel fabrication.
  • To improve cell viability and proliferation within the microgels.

Main Methods:

  • Utilized an aqueous two-phase system (ATPS) composed of dextran (DEX) and polyethylene glycol (PEG).
  • Engineered a co-flow microfluidic device with a square-wave-changing injection force for droplet generation.
  • Employed horseradish peroxidase-catalyzed crosslinking of alginate and carboxymethyl cellulose derivatives.

Main Results:

  • Successfully prepared uniform cell-laden microgels with controllable diameters using the ATPS.
  • Achieved high cell viability and proliferation within the microgels.
  • Demonstrated efficient microgel formation without oil-phase complications.

Conclusions:

  • The ATPS microfluidic method offers an efficient and effective alternative to oil-based systems for producing cell-laden microgels.
  • This approach enhances cell encapsulation and viability for biomedical applications.
  • The developed method provides precise control over microgel properties and production.