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Related Experiment Videos

Sympathetic ablation alters lymphocyte membrane properties.

K Miles, E Chelmicka-Schorr, S Atweh

    Journal of Immunology (Baltimore, Md. : 1950)
    |August 1, 1985
    PubMed
    Summary

    Peripheral sympathetic nervous system ablation alters spleen lymphocyte populations. Beta-adrenergic receptor density and specific cell surface antigens (Thy-1.2, Lyt-2) change, impacting immune cell interactions.

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    Area of Science:

    • Immunology
    • Neuroscience
    • Cell Biology

    Background:

    • The peripheral sympathetic nervous system (SNS) innervates lymphoid organs, including the spleen.
    • SNS activity can modulate immune cell function and distribution.
    • Understanding these neuro-immune interactions is crucial for comprehending immune responses.

    Purpose of the Study:

    • To investigate the impact of peripheral sympathetic nervous system ablation on spleen lymphocyte subpopulations.
    • To analyze changes in beta-adrenergic receptor density and specific cell surface antigens (Thy-1.2, Lyt-2) on lymphocytes following SNS denervation.

    Main Methods:

    • Peripheral sympathetic nervous system ablation was induced in mice using 6-hydroxydopamine hydrobromide (axotomy and sympathectomy).
    • Beta-adrenergic receptor density was quantified using radioligand binding assays (3H-dihydroalprenolol).

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  • Lymphocyte subpopulations expressing Thy-1.2 and Lyt-2 surface antigens were identified and quantified using fluorescent monoclonal antibodies and fluorescence-activated cell sorting (FACS).

    • Main Results:

    • Beta-adrenergic receptor density was higher on splenic B cells than T cells in control mice.
    • SNS axotomy led to increased beta-adrenergic receptor density on both T and B spleen cells.
    • Axotomy increased the percentage of Thy-1.2 positive cells and Lyt-2 positive cells within the total spleen lymphocyte population.
    • Sympathectomy did not alter the percentage of Thy-1.2 positive cells but decreased the representation of Lyt-2 positive cells within the Thy-1.2 positive population.

    Conclusions:

    • Peripheral sympathetic nervous system denervation significantly alters the distribution of spleen lymphocyte subpopulations and their beta-adrenergic receptor expression.
    • Changes in Thy-1.2 and Lyt-2 antigen expression suggest modulation of T cell subsets, potentially affecting immune cell function.
    • These findings highlight the intricate relationship between the sympathetic nervous system and lymphocyte physiology, influencing immune cell interactions and responses.