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[Quality Control and Biological Assessment of Automated Synthesized 3'-deoxy-3'-¹⁸F-fluorothymidine using CFN-MPS-200
Xiao Jiang1, Xiao-Xiong Wang1, Hao Lu1
1PET/CT Centre, Department of Radiology, Sichuan Cancer Hospital, Chengdu 610061, China.
Objectives:
To synthesize 3'-deoxy-3'-¹⁸F-fluorotyhymidine)(¹⁸F-FLT) using CFN-MPS-200 automatic synthesis module, and evaluate its distribution in Wistar rats.
Methods:
We used 3-N-Boc-5!d-O-dimethoxytrityl-3!d-O-nosyl-thymidine (Boc-FLT)-percursor as raw material to synthesize ¹⁸F-FLT without residual solvents. Its radiochemical purity was confirmed with radio-HPLC and thin layer chromatography (TLC). Normal Wistar rats were injected with 18 F-FLT and underwent PET scanning.
Results:
The entire preparation procedure took about 60 min, which resulted in a radio chemical yield of (24±5)% (after attenuation correction, n =20) and radiochemical purity of over 99%, with 1.11×10⁸ Bq/mL specific activity. The ¹⁸F-FLT solution was colorless and had a pH value between 7.0-8.0. ¹⁸F-FLT was mainly concentrated in the kidney, bladder, liver, bone marrow and Liver of normal Wistar rats.
Conclusion:
Automated synthesis of ¹⁸F-FLT using CFN-MPS-200 is a stable method, with high yield, safety without solvent, and acceptable quality.
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