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Related Experiment Videos

Rat serum contains a developmentally regulated cholinergic inducing activity.

E J Wolinsky, P H Patterson

    The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
    |June 1, 1985
    PubMed
    Summary

    Rat serum contains a macromolecular factor that induces sympathetic neurons to produce acetylcholine. This activity is age-dependent, appearing after 9 postnatal days, mirroring in vivo neurotransmitter status changes.

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    Area of Science:

    • Neuroscience
    • Developmental Biology

    Background:

    • Sympathetic neurons cultured in defined media lack acetylcholine production.
    • Serum supplementation enables acetylcholine production in cultured sympathetic neurons.

    Purpose of the Study:

    • To investigate the cholinergic-inducing activity present in rat serum.
    • To characterize the nature and developmental timing of this activity.

    Main Methods:

    • Culturing sympathetic neurons in defined media with varying serum concentrations.
    • Fractionation of serum to isolate active components.
    • Assessing acetylcholine production in cultured neurons.

    Main Results:

    • Cholinergic induction in sympathetic neurons is dependent on serum concentration.

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  • The inducing activity is associated with a macromolecular fraction of the serum.
  • Serum from rats younger than 9 postnatal days shows minimal cholinergic-inducing activity.

    • Conclusions:

    • Rat serum contains a macromolecular factor that induces cholinergic neurotransmitter synthesis in sympathetic neurons.
    • The developmental timing of this activity in vitro correlates with in vivo sympathetic nervous system development.
    • This factor may play a role in the developmental switch from noradrenergic to cholinergic function in sympathetic neurons.