Lateral Flow Aptasensor for Small Molecule Targets Exploiting Adsorption and Desorption Interactions on Gold...

Area of Science:

• Biotechnology
• Analytical Chemistry
• Biosensors

Background:

• Existing lateral flow assays (LFAs) are unsuitable for small molecule detection.
• A new aptamer-based LFA design is needed for small molecule sensing.

Purpose of the Study:

• To develop a novel lateral flow assay (LFA) for small molecule detection.
• To demonstrate the effectiveness of aptamer dissociation from gold nanoparticles for sensing.

Main Methods:

• Developed a new LFA by probing aptamer dissociation from gold nanoparticles.
• Utilized bovine serum albumin and lysozyme for test and control lines.
• Validated the sensor with 17β-estradiol and bisphenol A aptamers.

Main Results:

• Achieved nanomolar detection levels for small molecules.
• Demonstrated excellent selectivity and robust performance in natural water samples.
• Successfully detected 17β-estradiol and bisphenol A.

Conclusions:

• The novel LFA platform is effective for small molecule detection.
• The sensor design is versatile and applicable to various aptamers and targets.
• This technology offers a simple, rapid, and cost-effective diagnostic tool.