Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

58.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.3K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.3K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.3K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[DNA informs on ancient partner choice].

Medecine sciences : M/S·2026
Same author

[TimeVaults provide recordings of past cellular activity].

Medecine sciences : M/S·2026
Same author

[In vivo CAR-Ts, a significant breakthrough].

Medecine sciences : M/S·2026
Same author

[House of cards?]

Medecine sciences : M/S·2026
Same author

[Towards gene therapy for Hutington's disease].

Medecine sciences : M/S·2025
Same author

[DNA reveals the living world].

Medecine sciences : M/S·2025
Same journal

[CREPT: An ally of immunotherapy for the fight against non-small-cell lung cancers].

Medecine sciences : M/S·2026
Same journal

[A new regulatory mechanism of HIF-1α: the ROS-ATM-CHK2 axis at the core of tumor angiogenesis].

Medecine sciences : M/S·2026
Same journal

[Vibrio-host interactions: from the environmental niche to virulence].

Medecine sciences : M/S·2026
Same journal

[RcsF, hyperconnected sentinel of the bacterial envelope].

Medecine sciences : M/S·2026
Same journal

[Single-cell RNA-sequencing: shattering the clone myth].

Medecine sciences : M/S·2026
Same journal

[No, Physarum blobs do not have 720 sexes!]

Medecine sciences : M/S·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.2K

[A new milestone in human embryo editing].

Bertrand Jordan1

  • 1UMR 7268 ADÉS, Aix-Marseille, Université/EFS/CNRS, Espace éthique méditerranéen, hôpital d'adultes la Timone, 264, rue Saint-Pierre, 13385 Marseille Cedex 05, France ; CoReBio PACA, case 901, parc scientifique de Luminy, 13288 Marseille Cedex 09, France.

Medecine Sciences : M/S
|June 15, 2017
PubMed
Summary
This summary is machine-generated.

Scientists have successfully used CRISPR-Cas9 gene editing to correct a disease mutation in viable human embryos. While imperfect and not implanted, this marks a step toward human germline editing and its ethical considerations.

More Related Videos

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.6K
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.2K

Related Experiment Videos

Last Updated: Feb 28, 2026

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.2K
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.6K
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.2K

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioethics

Background:

  • CRISPR-Cas9 gene editing technology offers potential for correcting genetic diseases.
  • Human germline editing remains a contentious area due to ethical and safety concerns.

Purpose of the Study:

  • To investigate the efficacy of CRISPR-Cas9 in correcting a specific disease mutation in human embryos.
  • To assess the feasibility of gene editing in early human development.

Main Methods:

  • CRISPR-Cas9 gene editing was applied to viable human embryos.
  • Embryos were analyzed for successful gene correction and off-target effects.

Main Results:

  • Correction of the targeted disease mutation was achieved in the human embryos.
  • The gene correction process was not entirely perfect, with some residual imperfections observed.

Conclusions:

  • This study demonstrates the potential of CRISPR-Cas9 for correcting disease-causing mutations in human embryos.
  • The findings advance the discussion on the future of human germline gene editing and its ethical implications.