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Chemotaxis by Entamoeba histolytica.
The Journal of Protozoology
|May 1, 1985
Summary
Entamoeba histolytica exhibits chemotaxis towards bacteria and certain soluble molecules. This study developed a micropore membrane assay to investigate these responses, revealing attractants in bacterial suspensions and rat colon washings.
Area of Science:
- Microbiology
- Cell Biology
- Parasitology
Background:
- Entamoeba histolytica is an important human pathogen responsible for amoebiasis.
- Understanding the mechanisms of Entamoeba histolytica motility and host interaction is crucial for developing effective treatments.
Purpose of the Study:
- To develop and validate a micropore membrane assay for quantifying chemotaxis in Entamoeba histolytica.
- To identify specific substances and environmental factors that elicit a chemotactic response in Entamoeba histolytica trophozoites.
Main Methods:
- A micropore membrane assay using polycarbonate membranes was employed to measure trophozoite migration.
- Checkerboard analysis was utilized to confirm chemotaxis.
- Responses to various chemoattractants including soluble substances, bacterial suspensions, and rat colon washings were assessed.
Main Results:
- Entamoeba histolytica trophozoites demonstrated significant migration towards bacterial suspensions (all seven species tested) and specific soluble substances like Trypticase and N-acetylneuraminic acid from egg mucin.
- Chemotaxis was confirmed by checkerboard analysis.
- A diminished but present response was observed towards filter-sterilized rat colon washings, suggesting both soluble and particulate attractants.
Conclusions:
- A reliable micropore membrane assay for Entamoeba histolytica chemotaxis was established.
- Entamoeba histolytica is attracted to whole bacteria, certain soluble molecules, and components within rat colon washings.
- These findings provide insights into the environmental cues guiding Entamoeba histolytica during host invasion.