Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-innocent Spectators: Decoupling the Role of Cations and Anions in Modulating Nanoparticle Synthesis.

Journal of the American Chemical Society·2026
Same author

Peak2Patch: High-Fidelity Functional Group Identification through Attention-Based Fusion of Infrared and Mass Spectra.

ACS omega·2026
Same author

Isolated and H<sub>2</sub>-reduced Anderson clusters catalyse low-temperature hydrogenation of CO<sub>2</sub> to methanol.

Nature chemistry·2026
Same author

La<sup>3+</sup> Networks and Speciation in the Molten State: Impact of Spacer Salt Selection on Structural Heterogeneity.

Journal of the American Chemical Society·2026
Same author

Realizing Cocktail Effects in Catalytic High-Entropy Metal-Organic Frameworks (HEMOFs) via Predictive Density of State Calculations.

Angewandte Chemie (International ed. in English)·2026
Same author

Tuning SF<sub>6</sub> affinity <i>via</i> tailoring pore environments in metal-organic frameworks.

Chemical communications (Cambridge, England)·2025

Related Experiment Video

Updated: Feb 28, 2026

Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks
04:53

Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks

Published on: May 26, 2023

1.8K

Multifunctional, Tunable Metal-Organic Framework Materials Platform for Bioimaging Applications.

Dorina F Sava Gallis, Lauren E S Rohwer, Mark A Rodriguez

  • 1X-ray Science Division, Advanced Photon Source, X-ray Science Division, Argonne National Laboratory , Argonne, Illinois 60439, United States.

ACS Applied Materials & Interfaces
|June 15, 2017
PubMed
Summary
This summary is machine-generated.

Novel metal-organic frameworks (MOFs) offer tunable near-infrared emission for bioimaging. These biocompatible materials demonstrate cell permeability and long-term fluorescence in live cells, paving the way for tracking studies.

Keywords:
bioimagingemissionlanthanidemetal−organic frameworknear-infrared

More Related Videos

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks
10:13

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks

Published on: April 28, 2023

3.2K
Synthesis and Characterization of Functionalized Metal-organic Frameworks
11:27

Synthesis and Characterization of Functionalized Metal-organic Frameworks

Published on: September 5, 2014

49.3K

Related Experiment Videos

Last Updated: Feb 28, 2026

Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks
04:53

Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks

Published on: May 26, 2023

1.8K
A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks
10:13

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks

Published on: April 28, 2023

3.2K
Synthesis and Characterization of Functionalized Metal-organic Frameworks
11:27

Synthesis and Characterization of Functionalized Metal-organic Frameworks

Published on: September 5, 2014

49.3K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Metal-organic frameworks (MOFs) are advanced porous materials with diverse applications.
  • Tunable emission properties are crucial for advanced bioimaging techniques.
  • Developing biocompatible imaging agents with near-infrared (NIR) emission is a key challenge in biomedical research.

Purpose of the Study:

  • To develop a novel multifunctional metal-organic framework (MOF) platform with tunable emission properties for in vivo bioimaging.
  • To evaluate the biocompatibility, cell permeability, and long-term stability of these MOF materials in live cells.
  • To demonstrate the utility of these MOFs as optical bioimaging agents using hyperspectral confocal fluorescence microscopy.

Main Methods:

  • Synthesis of multifunctional MOF materials with varying metal identities (Eu, Nd, Nd/Yb) and tunable porosity.
  • Characterization of emission properties spanning the deep red to NIR spectral region (614-1350 nm).
  • In vitro studies involving the synthesis of a nanoscale Eu-MOF analog, assessment of cell permeability in RAW 264.7 and HeLa cells, and evaluation of structural integrity in aqueous buffers.
  • Utilizing hyperspectral confocal fluorescence microscopy for efficient discrimination between MOF emission and cellular autofluorescence.
  • Long-term monitoring of MOF fluorescence within live cells for up to 48 hours.

Main Results:

  • A novel MOF platform exhibiting porosity and tunable emission (614-1350 nm) based on metal identity (Eu, Nd, Nd/Yb) was successfully developed.
  • The MOF materials demonstrated minimal toxicity to living cells and maintained structural integrity in water and phosphate-buffered saline.
  • The nanoscale Eu-MOF analog proved cell-permeable in RAW 264.7 and HeLa cells.
  • Hyperspectral confocal fluorescence microscopy enabled clear differentiation between Eu-MOF emission and cell autofluorescence.
  • The intrinsic emission of the fluorophore-based MOF was conserved in live cells for up to 48 hours, a first for such materials.

Conclusions:

  • The developed MOF materials are minimally toxic, structurally stable in biological media, and possess tunable NIR emission properties, making them suitable for bioimaging.
  • The cell-permeability and long-term fluorescence retention in live cells validate the biocompatibility and potential of these MOFs for advanced applications.
  • This study establishes a promising new class of optical bioimaging agents for long-term tracking and biodistribution studies in biological systems.