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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Dissecting the human microbiome with single-cell genomics.

Andrew C Tolonen¹, Ramnik J Xavier^2,3,4,5

¹Broad Institute of MIT and Harvard University, Cambridge, MA, 02142, USA. atolonen@broadinstitute.org.

Genome Medicine

|June 16, 2017

Summary

This summary is machine-generated.

Single-cell genome sequencing helps identify functions of uncultured human microbiome members. This method reveals genetic differences between strains and aids in studying disease-causing bacteria.

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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Area of Science:

Microbiology
Genomics
Human Microbiome Research

Background:

Culturing challenges limit understanding of many human microbiome members.
Single-cell genome sequencing offers a new avenue for microbial analysis.

Discussion:

This technique allows functional role assignment for previously uncultured microbes.
It provides insights into the genomic underpinnings of strain-level phenotypic variation.
Applications include targeted studies of immunogenic bacteria relevant to disease.

Key Insights:

Genome sequencing of single microbial cells is a powerful tool.
Functional roles of uncultured microbes can now be determined.
Genomic basis of phenotypic variation is elucidated.

Outlook:

Further application in understanding complex microbial communities.
Potential for developing novel diagnostics and therapeutics for microbiome-associated diseases.
Advancing the study of host-microbe interactions in health and disease.