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Published on: February 27, 2020
[HER2 promotes cell migration via inhibiting the expression of E-cadherin in human mammary epithelial cells]
Shan Liang1, Zichuan Jiang2, Huan Liu2
1Life Science and Technology Institute, Yangtze Normal University, Chongqing 408100, China. *Corresponding author,
Abstract:
Objective To study the effect of HER2 on morphology and migration of MCF10A human mammary epithelial cells and its mechanism. Methods The stable HER2-overexpressing MCF10A cells were established by lentivirus infection. Then, cell migration was analyzed by TranswellTM assay; E-cadherin expression was detected by Western blotting, real-time quantitative PCR and immunofluorescent staining. Results HER2-overexpressing MCF10A cells showed scattering growth pattern and increased migration ability. Overexpression of HER2 decreased the expression of E-cadherin protein in MCF10A cells, and also reduced the membrance-associated E-cadherin. In addition, HER2-induced cell scattering and migration could be significantly suppressed by re-expression of E-cadherin. Conclusion HER2 promotes the migration of human mammary epithelial cells by inhibiting the expression of E-cadherin.
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