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Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and

Yu-Chuan Lin1, Yeukuang Hwu2, Guo-Shu Huang3

  • 1Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.

Scientific Reports
|June 16, 2017
PubMed

Insights

High-resolution 3D imaging identified novel markers for mouse kidney disease, differentiating tubulointerstitial lesions and glomerulopathy. These markers correlate with microvascular changes, aiding CKD diagnosis.

Area of Science:

  • Nephrology
  • Medical Imaging
  • Biomedical Engineering

Background:

  • Chronic kidney disease (CKD) involves complex renal injuries, including tubulointerstitial (TI) lesions and glomerulopathy.
  • Accurate differentiation of CKD subtypes is crucial for effective treatment and prognosis.
  • Current diagnostic methods may not fully capture the intricate microvascular alterations in CKD.

Purpose of the Study:

  • To establish distinct 3D imaging markers for mouse kidney disease using high-resolution synchrotron microtomography.
  • To characterize and differentiate renal TI lesions and glomerulopathy based on microvascular changes.
  • To correlate novel 3D imaging markers with histopathology and molecular markers of renal injury.

Main Methods:

  • Utilized high-resolution synchrotron microtomography for 3D imaging of mouse kidneys.
  • Employed two mouse models of CKD: unilateral ureteral obstruction (UUO) and focal segmental glomerulosclerosis (FSGS).
  • Correlated 3D imaging findings with histopathology and molecular markers (CD31, connective tissue growth factor, vascular endothelial growth factor).

Main Results:

  • Identified five unique 3D imaging markers indicative of altered renal microvasculature in CKD models.
  • Demonstrated that these markers can differentially reflect and distinguish between TI lesions and glomerulopathy.
  • Showed that individual markers characterize specific disease developments, while integrated use provides comprehensive information.

Conclusions:

  • Established a practical strategy for characterizing CKD-associated renal injuries using microangiography-based 3D imaging.
  • Highlighted the significant role of dysfunctional microvasculature in the pathogenesis of renal lesions.
  • Validated 3D imaging markers as valuable tools for differentiating CKD subtypes and assessing disease status.

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