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Published on: May 10, 2018
[A Study on the Feasibility to Assay Minipig Insulin Through Human Insulin Radioimmunoassay Kit]
Meng-Yu Yang1, Yun-Yi Gao1, Yan Li1
1Diabetic Foot Care Center, Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, China.
Objectives:
To investigate the feasibility of using human-specific insulin radioimmunoassay (Ins-RIA) kit to measure the concentrations of serum insulin in minipigs.
Methods:
The Serum samples (219) of Bama minipigs were collected. The concentrations of serum insulin in Bama minipigs were measured by Ins-RIA kit and porcine-specific insulin enzyme linked immunosorbent assay (Ins-ELISA) kit, respectively. The linear regression analysis was undertaken in a randomly selected 175 samples (80% of total samples) to establish predictive equations between the concentrations of serum insulin measured by Ins-ELISA and the concentrations of serum insulin measured by Ins-RIA. The equations were then cross-validated in the remaining 44 samples (20% of total samples) that had not been included in the regression analysis.
Results:
Measured insulin concentration was lower with the Ins-ELISA than that with the Ins-RIA [(15.32±15.50) μIU/mL vs. (32.31±21.74) μIU/mL, respectively, P<0.000 1]; The final predictive equation for the Ins-ELISA (μIU/mL) was equal to -7.29+0.70 × Ins-RIA(μIU/mL) ( R2=0.94). The differences between the predicted values and the actual measured values were 17.18%.
Conclusions:
Insulin values in Bama minipigs obtained from the Ins-ELISA and Ins-RIA are not equivalent and differ significantly. However, the insulin concentration by Ins-ELISA can be well estimated by Ins-RIA.
