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Updated: Feb 28, 2026

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
An LC-MS/MS method to determine vancomycin in plasma (total and unbound), urine and renal replacement therapy
Suzanne L Parker1, Yarmarly C Guerra Valero1, Jenny L Ordóñez Mejia1
1Burns, Trauma & Critical Care Research Centre, UQ Centre for Clinical Research, Brisbane, Australia.
Aim:
Critical illness and medical interventions, such as renal replacement therapy, can cause changes to vancomycin pharmacokinetics and lead to suboptimal dosing. To comprehensively characterize vancomycin pharmacokinetic a method must measure vancomycin in a range of clinical matrices.
Results:
A LC-MS/MS method was developed using hydrophilic interaction liquid chromatography and microsample volumes, where possible. For all matrices, the linear concentration range was 1-100 μg/ml, interassay accuracy and precision was within 15%, and recovery above 80%. No matrix effects were observed. Calibration equivalence may be applied for some matrix combinations.
Conclusion:
A method for the analysis of vancomycin in plasma (total, unbound), urine and renal replacement therapy effluent, suitable for use in any patient pharmacokinetic study, has been developed and validated.
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