Three-dimensional structures of the vestibular sensory epithelia

Vestibular neurosensory epithelia of the guinea pig and the bull frog were investigated by scanning electron microscopy. The crista ampullaris or macula were freeze fractured followed by maceration with 0.1% OsO4 solution for 24-60 h (osmium-DMSO-osmium method). Following this, three-dimensional intracellular structures were observed. The mitochondria which exist in the nerve chalice surrounding the type I cell were various in shape, globular, long and slender. Golgi apparatus, endoplasmic reticulum and thin fibrous structures of the sensory cells or supporting cells were clearly demonstrated. Concerning nervous system, nerve fibre, afferent and efferent nerve endings, further synaptic structures were also observed stereoscopically.