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In vivo endoscopic mass spectrometry using a moving string sampling probe.

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This study introduces an in situ endoscopic mass spectrometry system using a novel moving string probe. This innovation enables real-time analysis directly within the body, advancing diagnostic capabilities.

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Area of Science:

  • Analytical Chemistry
  • Medical Devices
  • Biotechnology

Background:

  • Current endoscopic analysis relies on ex situ biopsy and optical microscopy.
  • Limitations exist in real-time molecular analysis during endoscopy.
  • Need for minimally invasive, in situ diagnostic tools.

Purpose of the Study:

  • To develop and demonstrate a novel in situ and in vivo endoscopic mass spectrometry system.
  • To enable direct molecular analysis during endoscopic procedures.
  • To overcome limitations of traditional ex situ biopsy analysis.

Main Methods:

  • Construction of a prototype mass spectrometric endoscope integrating an industrial endoscope and commercial mass spectrometer.
  • Development of a sampling system using a moving cotton thread for material collection and transport.
  • Utilization of desorption electrospray and atmospheric pressure chemical ionization for sample ionization.
  • Demonstration of non-destructive analysis without heat, solvents, or high voltage.

Main Results:

  • Successful construction and testing of a prototype endoscopic mass spectrometer.
  • Demonstration of in situ endoscopic mass spectrometry on a living mouse.
  • Successful surface analysis within a volunteer subject's mouth.
  • Validation of the moving string probe for sample collection and transport.

Conclusions:

  • The developed system enables direct, in situ molecular analysis during endoscopy.
  • This technology offers a minimally invasive alternative to traditional biopsy methods.
  • Potential for enhanced diagnostic capabilities in various medical fields.